Presence of mast cells in the rat pericranium – a tissue very sensitive to pain
DOI:
https://doi.org/10.48208/HeadacheMed.2023.13Keywords:
Mast cells, Pericranium, Rats, Dura materAbstract
Objective
To evaluate mast cell presence in the pericranium of Wistar rats.
Methods
Five male rats of the Wistar strain were used. The animals were housed under a 12 h light cycle with ad libitum access to food and water and allowed 10 days of acclimatization before tissue sampling. The five rats were anesthetized by intraperitoneal injection of ketamine/xylazine, 10/20 mg/kg. Following aseptic preparation of the head skin, a midline longitudinal incision was made to expose the pericranium. Two samples of the pericranium were taken, one from the right and one from the left. These samples were fixed in 10% buffered formaldehyde for 24 h. After fixation, tissue samples were paraffin-embedded and sectioned at 4 μm. Then, slides were deparaffinized, stained with a concentration of 0.1% toluidine blue for 1 min, and washed with distilled water. Last, slides were photomicrographed under 400x magnification to identify mast cells.
Results
Mast cells were identified in the dura mater and the five rats' pericranium. In the dura mater, mast cells were also found in these rats. We found both granulated (intact) and degranulated mast cells.
Conclusion
We suggest that future preclinical studies investigating the involvement of dural mast cells and other meningeal cell populations should also include pericranium samples to explore this structure's relevance in migraine pain and other headache disorders.
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Copyright (c) 2023 Rita Santana dos Reis, Regina Lúcia Gomes Botter , Maria Rosana de Souza Ferreira, Sandra Lopes de Souza, Juliana Ramos de Andrade, Sarah Louise Tangsgaard Christensen, Marcelo Moraes Valença (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
