Headache Medicine 2021, 12(4):349 p-ISSN 2178-7468, e-ISSN 2763-6178

349

ASAA

DOI: 10.48208/HeadacheMed.2021.60

Headache Medicine

Thesis

A new anatomical-functional model: effect of topiramate on

capsaicin-induced mast cell degranulation in rat dura mater

(Abstract)

Um novo modelo anatômico-funcional: efeito do topiramato sobre a degranulação de mastócito induzida por

capsaicina na dura-mater de rato (Resumo)

Raisa Ferreira Costa

Dissertation (Master’s Degree in Biotechnology). Postgraduation in Biological Sciences. Federal University of Pernambuco, Recife, Brazil. 2020. 64 f.

Supervisor: Prof. Dr. Marcelo Moraes Valença.

Introduction

Migraine is an extremely disabling neurological disease due to its related symptoms, but the

pathophysiological mechanisms involved in the activation of a migraine crisis are still unknown.

Experimental models mimic the pathophysiology through chemical and electrical stimulations. Topiramate

is an important drug used to prevent migraine, but its mechanism of action is still not well known. A sterile

inammation of the dura mater is believed to trigger pain in the migraine.

Objective

Understand the effect of topiramate on sterile inammation of the dura mater that triggers migraine.

Method

We created an experimental model for the action mechanisms of topiramate and capsaicin in mast

cell degranulation. 35 male (n=28) and one group of females (n=7) 60-day-old rats were divided in 2

groups: topiramate (20mg/kg/day, gavage/10 days) and topiramate in situ on the dura mater (10

-3

20µl). The animals were anesthetized and cranial windows between the suture coronal and the lambda

in the hemicraniums were performed with a drill, to bilaterally expose the dura mater. Capsaicin 10

-3

M (80μl) was placed on the right side and synthetic interstitial uid (SIF) (80 μl) on the left side. The

percentage of degranulated mast cells was quantied after removal of the dura mater, by staining

toluidine blue (0.1%). Statistical analysis was performed using the Kolmogorov-Smirnov test, t-test and

ANOVA.

Results

There was a greater amount of degranulated mast cells in the dura mater of the hemicranium stimulated

with capsaicin when compared to the contralateral control side, both in females (18.43 ± 3,00% versus

73.11 ± 2.67%; p = 0.001) and in males (27.21 ± 1.91% versus 75.00 ± 2.55%; p < 0.001) as there was

no statistic, we used only males. In the group treated with topiramate for 10 days, there was a smaller

amount of degranulated cells by capsaisin (22.80 ± 1.17% versus 77.00 ± 1.32%, p < 0.001). The

topiramate placed in situ concomitantly with capsaicin also reduced the mast cell degranulation process

in the dura mater (35.74 ± 1.69% control versus 44.52 ± 0.82% capsaicin + topiramate; p < 0.001).

Conclusion

This study was able to demonstrate that capsaicin is a chemical method of inducing and stimulating mast

cells and that topiramate reduces the effect of capsaicin.

raissac0sta@hotmail.com

Received: January 20, 2022

Accepted: January 25,2022

Keywords:

Topiramate

Mast cell

Dura mater

Rat

Capsaicin